Table 2 Plasmids used in this study
Name | Characteristics | Origin |
|---|---|---|
pBOL199 | Delivery vector, p426-TDH3p-eutE | [36] |
pMEL11 | 2 μm ori, amdS, SNR52p-gRNA.CAN1.Y-SUP4 t | [33] |
pROS11 | amdS, gRNA.CAN1-2 μm ori-gRNA.ADE2 | [33] |
pUDE197 | 2 μm ori, p426-TDH3p-eutE-CYC1t | This work |
pUDI076 | pRS406-TDH3p-eutE-CYC1t | This work |
pUDR122 | 2 μm ori, amdS, SNR52p-gRNA.GND2.Y-SUP4 t | This work |
pUDR123 | 2 μm ori, amdS, SNR52p-gRNA.GND1.Y-SUP4 t | This work |
pMK-RQ-gndA | Delivery vector, TPI1p-gndA-CYC1 t | GeneArt, Germany |
pMK-RQ-6pgdH | Delivery vector, TP1Ip-6pgdh-CYC1 t | GeneArt, Germany |
pMK-RQ-gox1705 | Delivery vector, TPI1p-gox1705-CYC1 t | GeneArt, Germany |