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Fig. 4 | Microbial Cell Factories

Fig. 4

From: Deregulation of S-adenosylmethionine biosynthesis and regeneration improves methylation in the E. coli de novo vanillin biosynthesis pathway

Fig. 4

Effect of metJ deletion on protocatechuate and vanillate titers (a) in different host strains and (b) in the presence of amino acid supplementation. For these experiments, the following genes were overexpressed: aroG*,asbF Bt , OMT Hs , ppsA, and tktA. For the amino acid supplementation experiment (c), 10 mM of amino acid was added at induction. d Effect of overexpressing feedback-desensitized variants of metA and cysE along with aroG*,asbF Bt , OMT Hs , ppsA, and tktA in the RARE ∆metJ host. The control represents co-transformation with an empty pCOLADuet-1 plasmid

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Microbial Cell Factories

ISSN: 1475-2859

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