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Fig. 2 | Microbial Cell Factories

Fig. 2

From: Deregulation of S-adenosylmethionine biosynthesis and regeneration improves methylation in the E. coli de novo vanillin biosynthesis pathway

Fig. 2

Expression and activity data suggests principal limitation is not heterologous O-methyltransferase. a SDS-PAGE gel suggesting robust OMT Hs expression in soluble fractions of cells sampled from flask cultures. b In vitro specific activity measurements normalized to activity at 12 h time point. Activity data indicates that OMT Hs activity declines from 12 to 48 h. However, notable activity even at 48 h strongly suggests that loss of OMT Hs activity is not responsible for observed reduction in rate of vanillate formation

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Microbial Cell Factories

ISSN: 1475-2859

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