Table 3 Bacterial strains used and generated in this study
Bacterial strains | Relevant properties | Source of reference |
|---|---|---|
B. subtilis strains | ||
168 | trpC2 | UMCG, laboratory stock |
168 HmB C5 | trpC2 P(htrB)-gfp::spc | [35] |
168 ∆rok | trpC2, ∆rok::cm | UMCG, laboratory stock |
168degUhy32 | trpC2, degUhy32::kan | UMCG, laboratory stock |
DB104 | His nprR2 nprE18 ∆aprA3 | [36] |
168 HmB C5::pKTH10 | 168 HmB C5 carrying plasmid pKTH10 | This study |
168 HmB C5::pDAM | 168 HmB C5 carrying plasmid pDAM | This study |
168::pDAPamy-gfp | 168 carrying plasmid pDAPamy-gfp | This study |
168::pDAPamy-gfp tet | 168 carrying plasmid pDAPamy-gfp tet | This study |
168degUhy32::pDAPamy-gfp tet | 168degUhy32 carrying plasmid pDAPamy-gfp tet | This study |
168∆rok::pDAPamy-gfp tet | 168∆rok carrying plasmid pDAPamy-gfp tet | This study |
168::pDAamyM-gfp | 168 carrying plasmid pDAamyM-gfp | This study |