Table 2 Plasmids and strains used in this study

pET-30a

Novagen

T7 promoter, T7 terminator

pE194

[18]

Erythromycin resistance gene (ermE)

pCD256

[43]

Low copy plasmid in L. plantarum

pCDLbu1

[15]

High copy plasmid in L. plantarum

pCDLbu1_P_T7_mCherry_T_T7_Ery

This study

T7 RNA polymerase specific promoter

pCDLbu1ΔEc_P_T7_mCherry_T_T7_Ery

This study

T7 RNA polymerase specific promoter, without sequences for replication and selection in E. coli

pCDLbu1ΔEc_P₁₁_mCherry

[47]

pCD256_P_lacSynth_mCherry

This study

Low copy plasmid; promoter P_lacSynth; gene of interest mCherry

IPTG (1 mM)

pCD256_P_lacSynth_T7RNAP

This study

Low copy plasmid; promoter P_lacSynth; gene of interest T7 RNA polymerase

IPTG (1 mM)

pCDLbu1_P_lacSynth_mCherry

This study

High copy plasmid; promoter P_lacSynth; gene of interest mCherry

IPTG (1 mM), TMG (17 mM)

pCDLbu1_P_lacA_mCherry

This study

High copy plasmid; promoter P_lacA; gene of interest mCherry

Lactose (0.5 -2 % w/v)

pCDLbu1_P_xylA_mCherry

This study

High copy plasmid; promoter P_xylA; gene of interest mCherry

Xylose (0.2 -2 % w/v)

pCDLbu1_P_{xylA(nativeRBS)}_mCherry

This study

High copy plasmid; promoter P_xylA; gene of interest mCherry; native RBS and spacer sequence from B. megaterium DSMZ 319 xylA

Xylose (2 % w/v)

Strains

B. megaterium DSMZ 319

DSMZ

E. coli Neb10β

NEB

L. plantarum CD033

[43]

L. plantarum 3NSH

[17]

Plasmid cured L. plantarum CD033