Fig. 6

Promoter characteristics and analysis of mCherry expression under control of P_T7, regulated by T7 RNA polymerase under control of P_lacSynth, and growth behavior. a Change of mCherry expression (RFUs, relative fluorescent units) over time (hours) regulated by T7 RNA polymerase dual plasmid system in BioLector^® micro-fermentation is shown. T7 RNAP is under control of P_lacSynth and induced with 1 mM IPTG (or non-induced) after 2 h. MRS medium with glucose either 5 μg ml⁻¹ CM or 5 µg ml⁻¹ Ery (or both for the dual plasmid system). –x-: induced; mean values of four replicates are given and standard deviations are indicated. –: non-induced; mean values of three replicates are given and standard deviations are indicated. b Calculated OD₆₀₀ values. Solid line negative control (n = 3; pCDLbu1ΔEc_P_T7_mCherry_T_T7_Ery)