Fig. 1
From: Enhancing full-length antibody production by signal peptide engineering
Electron microscopy of E. coli expressing full-length hu5D5. a–b Transmission electron microscopy (TEM) images of 64B4 host strain expressing the empty vector pBR322 (a) or pBR-ssSTII0.3-ssSTII1-hu5D5 (b). White arrowheads point to inclusion bodies and black arrows point to the periplasm. c–d Immunostaining EM probing for light chain using anti-LC antibody (c) or heavy chain using anti-Fc antibody (d). Black arrows point to the periplasm. Black arrowheads point to gold particles indicating the cellular localization of light chain or heavy chain. Scale bars equal to 200 nm. The complete plasmid sequences were confirmed by DNA sequencing