Fig. 9

The kdpD460 mutant strain prevents the growth of competing strains during prolonged incubation in mixed culture. The strains tested as a minority were grown as monocultures in Pi-limiting medium for 1 day, diluted 10⁵-fold into 50 ml of 1-day-old cultures of the ancestral strain in Pi-limiting medium, and incubated further for 8 days. On day 9 of incubation, 1 ml of mixed culture was added into 50 ml of fresh Pi-limiting medium and incubated further for up to 17 days. The ancestral strain was ENZ1797 (ENZ535 Tc^r) when one evolved strain was added as a minority (a, b), and ENZ535 when two evolved strains were added as minorities (c, d)