Table 1 Comparison of growth rates and final OD600 readings between paired plate and L24 PTVA strains
From: Liquid PTVA: a faster and cheaper alternative for generating multi-copy clones in Pichia pastoris
Strain | Plate PTVA | Liquid PTVA | ||||||
|---|---|---|---|---|---|---|---|---|
Copy number | Yield (AU) | Growth rate | Final OD600 | Copy number | Yield (AU) | Growth rate | Final OD600 | |
pZGFP | 4 | 69,880 | 0.2973 | 59.2 | 4 | 66,829 | 0.4291 | 65.6 |
pZGFP | 10 | 211,853 | 0.31 | 46 | 10 | 187,676 | 0.4481 | 66.1 |
pZαGFP | 3 | 9308 | 0.3231 | 46.3 | 3 | 9128 | 0.46 | 57.3 |
pZαGFP | 7 | 6951 | 0.3228 | 48.2 | 7 | 6609.5 | 0.4283 | 61.7 |
pKGFP | 2 | 24,068 | 0.3447 | 57.9 | 2 | 27,367 | 0.3474 | 48.4 |
pKGFP | 6 | 49,650 | 0.3443 | 48.9 | 7 | 51,771 | 0.3426 | 47.7 |
pKαGFP | 3 | 4643 | 0.3335 | 48.1 | 3 | 4789 | 0.3529 | 50.9 |
pKαGFP | 7 | 2858 | 0.3491 | 51.1 | 8 | 4000 | 0.3472 | 47.0 |