Effect of cerulenin on fatty acid composition and gene expression pattern of DHA-producing strain Colwellia psychrerythraea strain 34H

Wan, Xia; Peng, Yun-Feng; Zhou, Xue-Rong; Gong, Yang-Min; Huang, Feng-Hong; Moncalián, Gabriel

doi:10.1186/s12934-016-0431-9

Table 2 Differential expression of genes involved in fatty acid metabolism in C. psychrerythraea without or with 12 μg/mL cerulenin at 10 °C

From: Effect of cerulenin on fatty acid composition and gene expression pattern of DHA-producing strain Colwellia psychrerythraea strain 34H

Locus ID	Gene ID	Gene description	Fold change (log₂)
Gene involved in fatty acid biosynthesis
CPS_RS06985	accA	acetyl-CoA carboxylase subunit A	0.28
CPS_RS04205	accB	acetyl-CoA carboxylase subunit B	0.47
CPS_RS04200	accC	acetyl-CoA carboxylase subunit C	0.39
CPS_RS17005	accD	acetyl-CoA carboxylase subunit D	−0.31
CPS_RS14645	fabA	3-hydroxydecanoyl-ACP dehydratase, isomerase	−0.21
CPS_RS17030	fabB	3-ketoacyl-ACP synthase I	0.96
CPS_RS10240	fabF	3-ketoacyl-ACP synthase II	0.41
CPS_RS09575	fabH	3-ketoacyl-ACP synthase III	0.38
CPS_RS10225	fabD	malonyl-CoA:ACPtransacylase	0.95
CPS_RS07145	fabG1	3-ketoacyl-ACP reductase	0.01
CPS_RS10230	fabG2	NADPH-dependent 3-ketoacyl-ACPreductase	−0.03
CPS_RS06960	fabZ	3-hydroxyacyl-ACP dehydratase	−0.06
CPS_RS01535	fabR	DNA-binding transcriptional repressor	−0.04
Gene involved in fatty acid degradation
CPS_RS01760	fadA	3-ketoacyl-CoA thiolase	−0.57
CPS_RS01765	fadB	3-hydroxyacyl-CoA dehydrogenase	−0.92
CPS_RS05300	fadD1	Long chain fatty acid-CoA ligase	−1.51
CPS_RS15290	fadD2	Long chain fatty acid-CoA ligase	0.28
CPS_RS10085	fadE	Acyl-CoA dehydrogenase	0.40
CPS_RS04110	fadH	2,4-dienoyl-CoA reductase	0.55
CPS_RS15575	fadR	Fatty acid metabolism regulator	−0.39
Gene involved in fatty acid modification or lipid metabolism
CPS_RS00390	cti	fatty acid cis/trans isomerase	0.11
CPS_RS10220	plsX	glycerol-3-phosphate acyltransferase	−0.30
CPS_RS19415	plsY	glycerol-3-phosphate acyltransferase	0.31
CPS_RS00605	GPAT	glycerol-3-phosphate acyltransferase	0.21
CPS_RS04525	LPAAT	1-acyl-sn-glycerol-3-phosphate acyltransferase	0.06
CPS_RS21310	LPAAT	1-acyl-sn-glycerol-3-phosphate acyltransferase	−0.08
CPS_RS04435		acyltransferase	0.02
CPS_RS08065		acyltransferase	0.25
CPS_RS14560		acyltransferase	0.55
CPS_RS21745		acyltransferase	0.52
CPS_RS22505		acyltransferase	0.70
CPS_RS22520		acyltransferase	−0.20
Genes involved in PKS pathway
CPS_RS13885	pfaA	polyunsaturated fatty acid synthase	−0.58
CPS_RS13880	pfaB	polyunsaturated fatty acid synthase	−0.37
CPS_RS13875	pfaC	polyunsaturated fatty acid synthase	0.03
CPS_RS13865	pfaD	polyunsaturated fatty acid synthase	0.60
CPS_RS13895	pfaE	4′-phosphopantetheinyl transferase	−0.51

Fold change (log₂ values) in transcript levels under specified conditions as determined by RNA-seq. Transcript abundance obtained from RNA-seq data is indicated as RPKMs (See “Methods” section)

Back to article page

ISSN: 1475-2859

Contact us

Submission enquiries: journalsubmissions@springernature.com

Microbial Cell Factories

Contact us