Fig. 3

Surface accessibility of CYP1A2 and CPR. a SDS-PAGE of outer membrane protein isolations and investigation of protease accessibility. M: Protein marker, apparent molecular weights are indicated on the left. Lane 1–2 sample from E. coli BL21(DE3) host cells, lane 3 sample from cells with induced expression of CPR fusion protein, lane 4 sample from cells with induced expression of CYP1A2 fusion protein, lane 5–6 samples from cells with induced co-expression of both fusion proteins. Samples in lane 2 and 6 were treated with proteinase K prior to outer membrane protein isolation. b Flow cytometry analyses of immunolabelled cells. Cell samples were treated with a primary monoclonal anti-myc antibody and a secondary Dylight488 conjugated anti-IgG antibody, washed and then analyzed via flow cytometry. Grey E. coli BL21(DE3) control cells, red cells expressing CYP1A2, orange cells expressing CPR