From: New approaches to achieve high level enzyme production in Streptomyces lividans
Plasmid | Characteristics | Reference |
---|---|---|
pN702GEM3 | E. coli–Streptomyces shuttle vector; contains the pIJ702 origin of replication (for Streptomyces), the pUC origin of replication (for E. coli) and a polylinker derived from pGEM3Zf(+) (Promega). It carries the bifunctional Neo/Kan resistance marker from Tn5 (for both E. coli and Streptomyces) | [21] |
pNX30 | pN702GEM3 derivative. xysA xylanase ORF without any promoter and flanked by transcriptional terminators. Used as negative control | [35] |
pNX24 | pN702GEM3 derivative. xysA promoter from S. halstedii controls xylanase expression | [22] |
pNUF5 | pN702GEM3 derivative. pstS promoter from S. lividans controls xylanase expression | [20] |
pNErmX | pN702GEM3 derivative. Erythromycin resistance promoter from S. erythraeus controls xylanase expression | This work |
pNvsi | pN702GEM3 derivative. vsi promoter from S. venezuelae controls xylanase expression | This work |
pNX26 | pN702GEM3 derivative. Xylose isomerase promoter from S. coelicolor controls xylanase expression | This work |
pNXHid | pN702GEM3 derivate. Promoter of a putative hydrolase (glpQ) from S. coelicolor controls xylanase expression | This work |
pNXA1 | pNX24 derivate. xysA promoter from S. halstedii controls xylanase expression with amylase signal peptide | This work |
pNXA2 | pNX24 derivative. xysA promoter from S. halstedii. Controls xylanase expression with the amylase signal peptide with three additional amino acids | This work |
pNXAmy | pN702GEM3 derivative. xysA promoter from S. halstedii controls amylase expression | This work |
pNUFAmy | pN702GEM3 derivative. pstS promoter from S. lividans controls amylase expression | This work |
pEPOS101 | pHJL401 derivative harbouring xysAp driving the expression of SLAC (SCO6712) | This work |