Fig. 2
From: New approaches to achieve high level enzyme production in Streptomyces lividans
Xylanase and amylase production under the control of different promoters. a. Xylanase production by S. lividans 1326 transformed with different constructions (pNX30, pNX24, pNUF5, pNX26, pNXHid, pNErmX, and pNvsi) after 5 days of culture in YES medium supplemented with 1 % xylose. b. Xylanase production by S. lividans 1326 transformed with pNX24 in YE or YES media, both supplemented with 1 % xylose. c. Xylanase production by S. lividans 1326 transformed with pNUF5 in YE or YES media, both supplemented with 5 % fructose. d. Diagram of expression plasmids pNXAmy and pNUFAmy. e. Amylase production by S. lividans 1326 transformed with pNXAmy in YES medium supplemented with 1 % xylose and with pNUFAmy in YE medium supplemented with 5 % fructose. 10 µL of supernatant of 5 days cultures were loaded in each track. Arrows indicate the two bands of 57 and 50 kDa of amylase generated by an intracellular processing [45]