Efficient fermentative production of polymer-grade d-lactate by an engineered alkaliphilic Bacillus sp. strain under non-sterile conditions

Table 2 EPS, lactate, and byproduct formation in Bacillus sp. N16-5 wild-type and engineered strains

Strain	EPS (g/l)	Pyruvate (g/l)	Succinate (g/l)	Lactate (g/l)	Formate (g/l)	Acetate (g/l)
WT	1.99 ± 0.13	0.36 ± 0.01	0.71 ± 0.01	3.64 ± 0.00^a	0.33 ± 0.00	3.74 ± 0.04
∆ldh	2.21 ± 0.03	2.86 ± 0.20	1.21 ± 0.02	0.64 ± 0.06^a	0.40 ± 0.04	3.65 ± 0.16
∆ldh∆epsD	0.68 ± 0.03	2.88 ± 0.09	1.19 ± 0.02	0.77 ± 0.01^a	0.34 ± 0.02	3.56 ± 0.01
∆ldh-pDlac	2.09 ± 0.16	0.34 ± 0.01	0.46 ± 0.02	5.68 ± 0.23^b	0.12 ± 0.02	2.95 ± 0.12
∆ldh∆epsD-pDlac	0.62 ± 0.13	0.35 ± 0.02	0.52 ± 0.04	4.93 ± 0.22^b	0.07 ± 0.01	3.62 ± 0.08

Values were represented as the average ± standard deviation of three independent experiments
WT wild-type
^a l-lactate product
^b d-lactate product

ISSN: 1475-2859