Fig. 4
Biocatalytic process of the asymmetric oxidation of MOPE with Acetobacter sp. CCTCC M209061 strain in different reaction systems. Reaction conditions: (1) [C4MIM][PF6]/buffer biphasic system: 4 mL TEA-HCl buffer (100 mmol/L, pH 6.5); 1 mL [C4MIM][PF6]; 80 mmol/L MOPE; 0.3 g/mL immobilized beads; 122 mmol/L acetone; 30 °C; 220 rpm. (2) [C4MIM][PF6]/[ChCl][Gly]-buffer biphasic system:3.6 mL TEA-HCl buffer (100 mmol/L, pH 6.5); 1 mL [C4MIM][PF6]; 0.4 mL [ChCl][Gly]; 80 mmol/L MOPE, 0.3 g/mL immobilized beads; 122 mmol/L acetone; 30 °C; 220 rpm