Fig. 6

Co-overexpression of ME1 and GK in M. alpina. The expression level (a), enzymatic activity (b), NADPH level (c) and total fatty acid level (d) in M. alpina strains were analyzed after co-overexpressing ME1 and GK. M. alpina (black bars): wild type M. alpina; MA-malE1-gk-1 (striped bars): ME1 and GK co-overexpressing M. alpina strain. Strains were cultured in 500 mL shaking flask containing 200 mL Kendrick medium supplemented with 50 g/L glycerol for 168 h at 200 rpm. Three independent experiments were performed, and the error bars represent standard deviations. *p < 0.05 compared to wild type