Genome-scale metabolic reconstructions and theoretical investigation of methane conversion in Methylomicrobium buryatense strain 5G(B1)

de la Torre, Andrea; Metivier, Aisha; Chu, Frances; Laurens, Lieve M. L.; Beck, David A. C.; Pienkos, Philip T.; Lidstrom, Mary E.; Kalyuzhnaya, Marina G.

doi:10.1186/s12934-015-0377-3

Table 2 Computation predictions for different modes of methane oxidation and growth conditions

From: Genome-scale metabolic reconstructions and theoretical investigation of methane conversion in Methylomicrobium buryatense strain 5G(B1)

	Biomass flux^a	Oxygen consumption	O₂:CH₄ ratio	CO₂ production	CCE
Unconstrained network	0.246	21.34	1.16	7.78	57.85
Methane oxidation mode
Direct coupling	0.242	21.59	1.17	7.95	56.93
Direct coupling/low ATP maintenance	0.263	20.24	1.10	7.02	61.97
Direct coupling/low ATP yield	0.238	21.82	1.18	8.1	56.12
Low efficiency direct coupling, complemented by NADH (1 mol of NADH per 10 mol of methane oxidized)^b	0.23	21.43	1.16	8.47	54.12
Low efficiency direct coupling, complemented by NADH (1 mol of NADH per 5 mol of methane oxidized)	0.219	21.2	1.15	8.94	51.57
Uphill electron transfer/ NADH input (2/3 electrons from cytochrome cL and 1/3 from NADH)	0.224	21.8	1.18	8.72	52.76
Uphill electron transfer/NADH input (1/2 electrons from cytochrome cL and 1/2 from NADH)	0.205	22.99	1.25	9.53	48.37
Redox arm (0.5 mol ATP per 1 mol methanol oxidized)	0.146	27.69	1.50	12.13	34.29
Redox arm (1 mol ATP per 1 mol of methanol oxidized)	0.146	27.69	1.50	12.13	34.29
Redox arm (1 mol ATP per 1 mol of methanol oxidized)/low ATP maintenance	0.146	27.69	1.50	12.13	34.29
Redox arm (0.5 mol ATP per 1 mol methanol oxidized)/high ATP maintenance	0.142	27.92	1.51	12.29	33.42
Assimilation pathways (with low efficiency direct coupling complemented by NADH)^a
EMP only	0.232	21.28	1.15	8.37	54.66
EDD only	0.223	21.85	1.18	8.76	52.55
BS (acetyl-CoA synthesis only)	0.228	21.55	1.17	8.56	53.63
Serine cycle (acetyl-CoA synthesis only)	0.222	21.93	1.19	8.82	52.22
Nitrogen source
Nitrogen fixation	0.228	23.23	1.26	8.58	53.52
Ammonium	0.29	22.08	1.20	5.88	68.15
Urea^c	0.293	21.93	1.19	6.86	62.84
Sulfur source
HS⁻	0.233	21.35	1.16	8.37	54.66
HS⁻ and Urea^c	0.295	21.93	1.19	6.79	63.22

^aMethane uptake is 18.46 mmol g CDW ⁻¹ h⁻¹
^bMethane oxidation mode used to calculate data shown here for different sources of nitrogen and sulfur
^cOnly CO₂ from methane was used to calculate CCE

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ISSN: 1475-2859

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