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Table 1 Proteins identification by MALDI-TOF/TOF

From: Significantly improved solvent tolerance of Escherichia coli by global transcription machinery engineering

Spot no.

Homologous protein annotation

Coding gene

Mass (Da)

pI

Up/down regulation

Function

2512

Phosphotransferase enzyme family protein

UMNK88_2189

32,682

4.98

Unknown

5231

Universal stress global response regulator

uspA

16,028

5.08

Modulate and reorganize the carbon flow

5303

Conserved protein, UPF0070 family

yfgM

22,162

5.07

Unknown

5514

Galactose-binding transport protein

mglB

35,720

5.68

Galactose-binding transport

6204

Chain A, outer membrane protein

ompX

16,350

5.04

Promote bacterial adhesion

6224

Global DNA-binding transcriptional dual regulator H-NS

Hns

15,331

5.24

Global DNA-binding transcriptional dual regulator H-NS

6326

Chaperones protein HchA

hchA

31,190

5.63

HSP31 molecular chaperone

6328

Chain A, outer membrane protein Ompw

ompW

21,661

6.03

Acts as a receptor for colicin S4

6413

pspA Protein

pspA

25,562

5.51

Regulatory protein for phage-shock-protein operon

6437

Adenylate kinase

Adk

23,712

6.01

Energy metabolism

6524

Outer membrane protein OmpA

ompA

17,186

5.99

Serves as a receptor for a number of T-even like phage

6635

Tryptophanase

tnaA

53,098

5.88

Tryptophanase/l-cysteine desulfhydrase

7212

50S Ribosomal protein L9

Prll

15,759

6.15

RNA-binding protein

7303

Superoxide dismutase

soda

15,974

5.95

Destroys radicals in the cells

7315

ATP-dependent Clp protease, proteolytic subunit ClpP

Clp

23,286

5.52

Acts to disaggregate proteins

8419

Two-component system phosphate regulon response regulator OmpR

ompR

26,757

6.01

DNA-binding protein

7611

Aminopeptidase B

pepB

46,483

5.60

Probably acts in intracellular peptide degradation

8425

Succinate dehydrogenase iron-sulfur subunit

sdhB

27,394

6.31

Iron-sulfur protein subunit of succinate dehydrogenase

8524

Glyceraldehyde-3-phosphate dehydrogenase A

gapA

35,550

6.58

Encodes one of the two subunits of GapA

5404

Outer membrane porin protein C

ompC

40,343

4.58

Forms passive diffusion pores

4327

Thiol peroxidase

Bcp

17,995

5.03

Bacterioferritin comigratory protein

7703

Chain A, dipeptide transporter

dppA

57,599

5.74

Dipeptide-binding protein of a transport system that can be subject to osmotic shock

  1. ↑ Represents up-regulated genes; ↓ represents down-regulated genes