Fig. 1From: High-level production of membrane proteins in E. coli BL21(DE3) by omitting the inducer IPTGMembrane protein GFP fusions. In this study, membrane proteins were produced as C-terminal GFP fusions. The GFP moiety only folds properly and becomes fluorescent when the membrane protein-GFP fusion is inserted in the cytoplasmic membrane. When the membrane protein GFP fusion aggregates in the cytoplasm the GFP moiety does not fold properly and does not fluoresceBack to article page