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Table 2 Galactosylation and rhamnosylation potential of strains sGAL3 and sRHA3 respectively towards other flavonols

From: Metabolic engineering of Escherichia coli into a versatile glycosylation platform: production of bio-active quercetin glycosides

Flavonol

3-O-galactoside

3-O-rhamnoside

Cp (mg/L)

qp (mg/gCDW/h)

Cp (mg/L)

qp (mg/gCDW/h)

Kaempferol

84 ± 14

3.46 ± 0.86

416 ± 37a

12.1 ± 1.4

Myricetin

52 ± 7.1

2.88 ± 0.22

72.3 ± 9.1

2.8 ± 0.5

Morin

34 ± 5.8

1.65 ± 0.15

116 ± 21b

2.5 ± 1.5

Fisetin

134 ± 22

9.32 ± 0.55

403 ± 31c

11.3 ± 0.9

  1. Specific productivity (qp) and titer (Cp) reached after 48 h of incubation are shown
  2. ND not detected
  3. a3-O-glucoside was also detected at 52 ± 17 mg/L
  4. b3-O-glucoside was also detected at 21.7 ± 6.2 mg/L
  5. c3-O-glucoside concentration was lower than 5 mg/L
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Microbial Cell Factories

ISSN: 1475-2859

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