Microbial Cell Factories

Table 2 Summary of the evaluated expression systems for recombinant antibody production

From: Recombinant antibody production evolves into multiple options aimed at yielding reagents suitable for application-specific needs

Expression organism	Method robustness: specificities	References (for unusual organisms)
Escherichia coli	Prokaryotic gold standard, different methodologies	–
Lactobacillus paracasei	Effective diet therapy	[31, 36]
Lactobacillus rhamnosus	Effective diet therapy	[32]
Lactobacillus plantarum	Effective cargo in vivo	[46]
Bifidobacterium longum	Diet therapy to be demonstrated	[35]
Pseudoalteromonas haloplanktis	Protein folding at low temperature: high energy needs?	[37, 38]
Brevibacillus choshinensis	Highly productive for VHHs	[39–41]
Bacillus megaterium	No shown advantage	[42]
Corynebacterium glutamicum	No shown advantage	[43]
Pseudomonas putida	FDA biosafety certificate	[44]
Salmonella typhimurium	Suitable for display and in vivo cargo applications, biosafe	[45]
Pichia pastoris	Eukaryotic gold standard	–
Saccaromyces cerevisiae	Suitable for engineering	–
Schizosaccharomyces pombe	No shown advantage	[58]
CHO	Industrial gold standard: no shown advantage at research lab level	[63]
HEK293	No shown advantage at research lab level	[64]
Trichoderma reesei	Not thoroughly characterized	[61]
Aspergillus spp.	Not thoroughly characterized	[61]
Leishmania tarentolae	Favorable codon usage, low yields	[66, 67]
Trichoplusia ni	Positive preliminary results	[69]
Arabidopsis thaliana	Product heterogeneity	[73, 74]
Nicotiana benthamiana	Product heterogeneity	[75]
Oryza sativa	Optimized yields and quality	[91]
Physcomitrella patens	Suitable for photoreactor, promising for IgGs	[84–86]
Chlamydomonas reinhardtii	Promising for large immune-reagents	[87]

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ISSN: 1475-2859

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