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Table 1 Effect of over-production of GDH on 2,3-BD production in B. amyloliquefaciens

From: Enhanced 2,3-butanediol production from biodiesel-derived glycerol by engineering of cofactor regeneration and manipulating carbon flux in Bacillus amyloliquefaciens

Strains

CGly (g/L)

C Suc (g/L)

Specific activity of GDH (U/mg)

2,3-BD (g/L)

Acetoin (g/L)

Lactate (g/L)

Acetate (g/L)

Succinate (g/L)

DCW (g/L)

B10-127

80 ± 2

10 ± 1

0.12 ± 0.01

31.3 ± 1.03

8.56 ± 0.36

2.12 ± 0.10

1.11 ± 0.06

3.65 ± 0.15

9.21 ± 0.26

DH

80 ± 2

10 ± 1

0.69 ± 0.06

34.6 ± 1.12

4.87 ± 0.21

2.46 ± 0.12

1.02 ± 0.05

3.96 ± 0.17

9.12 ± 0.21

GL

80 ± 2

10 ± 1

0.48 ± 0.04

33.3 ± 1.15

6.65 ± 0.29

2.38 ± 0.12

1.04 ± 0.06

3.81 ± 0.16

9.16 ± 0.22

  1. C Gly glycerol consumption, C Suc sucrose consumption, GDH glycerol dehydrogenase
  2. Batch fermentation was carried out at 37 °C in 250-mL flasks [95 g/l co-substrate; molasses to crude glycerol ratio 1.5:8 (w/w)] at 37 °C and shaking speed 180 rpm
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Microbial Cell Factories

ISSN: 1475-2859

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