Figure 1

The deoxycytidine biosynthetic pathway. The steps engineered in this study are indicated by the bold arrows and lines. Components of the catabolic pathways are as follows: carAB carbamoyl phosphate synthase, pyrBI aspartate-carbamoyl transferase, pyrC dihydroorotase, pyrD dihydroorotate oxidase, pyrE orotate phosphoribosyl transferase, pyrF OMP decarboxylase, pyrG CTP synthetase, pyrH UMP kinase, yfbR dCMP phosphohydrolase, nrdCAB nucleotide diphosphate reductase, thyA thymidylate synthase, dcd dCTP deaminase, udk uridine kinase, deoA thymidine phosphorylase, tdk thymidine kinase, udp uridine phosphorylase, dut deoxyribonucleotide triphosphatase, ndk nucleotide diphosphate kinase, tmk TMP kinase, cdd cytidine deaminase, codA cytosine deaminase, deoD purine nucleoside phosphorylase, rihA, rihB ribosyl pyrimidine nucleosidase, cmk cytidylate kinase, purR purine repressor, pepA aminopeptidase A, argR arginine repressor.