Table 3 Strains and plasmids used in this research

Thr

The genome of this strain was used as the template for amplification of threonine synthesis genes

E. coli DH5a

This strain was used for the clone of the plasmid

E. coli BL21

This strain was used for enzyme protein expression

pET28a-thrA

N-terminal His-tagged thrA, inserted between NdeI and EcoRI sites of pET 28a (+)

pET28a-lysC

N-terminal His-tagged lysC, inserted between NdeI and EcoRI sites of pET 28a (+)

pET28a-asd

N-terminal His-tagged asd, inserted between NheI and EcoRI sites of pET 28a (+)

pET28a-thrB

N-terminal His-tagged thrB, inserted between NdeI and EcoRI sites of pET 28a (+)

pET28a-thrC

N-terminal His-tagged thrC, inserted between NdeI and BamHI sites of pET 28a (+)

pWSK29-lysC

lysC, inserted between EcoRI and BamHI sites of pWSK29