Figure 2From: Evaluation of genes involved in oxidative phosphorylation in yeast by developing a simple and rapid method to measure mitochondrial ATP synthetic activityThe effect of inhibitors on mitochondrial ATP synthesis in S. cerevisiae. Inhibitors of the mitochondrial respiratory chain: antimycin A, CCCP, and DCCD. To confirm the functionality of mitochondria, 0.1 mM antimycin A, 0.5 mM CCCP, and 0.1 mM DCCD were incubated with the crude mitochondria for 5 min before being subjected to ATP assay. The activities were shown in the relative values (%) compared with the value without inhibitor of respiratory chain as the control.Back to article page