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Table 1 Enzyme kinetic parameters of the purified r27RCLC, proRCLCNQ and its N-glycosylation mutants

From: Role of N-linked glycosylation in the secretion and enzymatic properties of Rhizopus chinensis lipase expressed in Pichia pastoris

Enzyme

k cat (s −1 )

K m (mM)

k cat / K m (M −1  s −1 )

r27RCLC

121.54 ± 3.1

0.34 ± 0.10

(3.56 ± 0.81)*105

proRCLCNQ

60.70 ± 2.2

0.20 ± 0.04

(2.92 ± 0.55)*105

proRCLCN14Q

24.82 ± 2.4

2.01 ± 0.12

(1.22 ± 0.15)*105

proRCLCN48Q

61.33 ± 2.5

0.19 ± 0.02

(3.11 ± 0.61)*105

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Microbial Cell Factories

ISSN: 1475-2859

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