From: Protein recovery from inclusion bodies of Escherichia coli using mild solubilization process
Solubilization method | Remarks | Reference |
---|---|---|
Tris-Cl buffer, low concentration of DMSO, n-propanol and Sarcosyl | Non-denaturing solubilization methods for non-classical inclusion bodies. No need of refolding | |
Low concentration of urea | Mild solubilization, low concentration of urea doesn’t completely denature solubilized protein molecules | |
Organic solvent based solubilization | High solubilization efficiency, inhibition of aggregation during refolding | [60] |
High hydrostatic pressure | High solubilization efficiency with simultaneous refolding of solubilized protein | |
High pH buffers | High pH in combination with low concentration of urea. Mild, efficient solubilization | |
Detergents | Mild solubilization |