Metabolic network analysis and experimental study of lipid production in Rhodosporidium toruloides grown on single and mixed substrates

Table 3 Elementary modes (EMs) and theoretical TAG yields with heterologous genes/reactions

Carbon source	EMs	Characteristics	Maximum yield of TAG [C-mol.C-mol ⁻¹ substrate]	Maximum yield of TAG [g.g ⁻¹ substrate]
Glucose	30,132	Transhydrogenase	0.64	0.33
Glycerol	57,172	Transhydrogenase	0.68	0.35
Xylose	21,323	Transhydrogenase	0.64	0.33
Arabinose	27,264	Transhydrogenase	0.64	0.33
Glucose	26,878	Cytosolic NADP-dependent GAPDH	0.62	0.32
Glycerol	42,578	Cytosolic NADP-dependent GAPDH	0.67	0.35
Glucose	6858	Without cytosolic Malic enzyme	0.51	0.27
Glucose	12,487	With NADP-dependent ALD6	0.58	0.30
Glycerol	23,658	With NADP-dependent ALD6	0.63	.032
Glucose	15,331	Cytosolic NADH source	0.68	0.36
Glycerol	35,804	Cytosolic NADH source	0.68	0.35

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