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Figure 1 | Microbial Cell Factories

Figure 1

From: Enhanced production of gamma-aminobutyrate (GABA) in recombinant Corynebacterium glutamicum by expressing glutamate decarboxylase active in expanded pH range

Figure 1

Comparison of GABA production in C. glutamicum producing wild type GAD or mutant GAD in flaks cultivation. (A) SDS-PAGE analysis of production of wild type GAD and mutant GAD. Lane M, protein size markers (kDa). Lanes 1 and 2, C. glutamicum harboring pCES208. Lanes 3 and 4, C. glutamicum harboring pHGwt. Lanes 5 and 6, C. glutamicum harboring pHGmut. Lanes 1, 3, and 5, total protein fractions. Lanes 2, 4, and 6, soluble protein fractions. The open and closed arrowheads indicate the band of wild type GAD and mutant GAD, respectively. (B) Time profiles of GABA concentration. Diamond and circle symbols indicate C. glutamicum harboring pHGwt and pHGmut, respectively.

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Microbial Cell Factories

ISSN: 1475-2859

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