Table 1 The bacterial strains and vectors used in the bioengineering of bacteria to produce higher alcohols
Strain or plasmid | Relevant genotype | Source |
|---|---|---|
Strains | ||
DH5α | F−, φ 80dlacZ ΔM15, Δ(lacZYA-argF )U169, deoR, recA1, endA1, hsdR17(rK −, mK +), phoA, supE44, λ −,thi −1, gyrA96, relA1 | Takara: 9057 |
C. crenatum | ompT, hsdSB (rB − mB − ),gal, dcm | CICC 20153 |
Plasmids | Â | Â |
pSTV29 | pACYC184 ori; Cmr; PLlacO−1: MCS | Takara: 3332 |
p29-pBL | pBL1ori; Cmr; PLlacO−1: MCS | This study |
PEC-XK99E | pGA1 Kmr pTrc99A MCS P-trc,lacIq | From CAS |
pXMJ19 | pBL1ori; Kmr; MCS P-trc, lacIq | From CAS |
PEC-KA | pGA1 Kmr pTrc99A MCS P-trc, lacIq: Kivd(LL)-ADH2(SC) | This study |
p29- AL | pBL1ori; Cmr; PLlacO-1:MCS:LEU2(SC) | This study |
p29- ALI | pBL1ori; Cmr; PLlacO-1: LEU2(SC)-ILV2(SC) | This study |
p29- ALII | pBL1ori; Cmr; PLlacO-1: LEU2(SC)-ILV2-ILV5(SC) | This study |
p29- BI | pBL1ori; Cmr; PLlacO-1: ILV2(SC) | This study |
p29- BII | pBL1ori; Cmr; PLlacO−1: ILV2(SC)-ILV5(SC) | This study |
p29- BIII | pBL1ori; Cmr; PLlacO−1: ILV2(SC)-ILV5(SC)-ILV3(SC) | This study |
p29- CB | pBL1ori; Cmr; PLlacO−1: BAT2(SC) | This study |
p29-DL | pBL1ori; Cmr; PLlacO−1: LEU4(SC) | This study |
p29-DLL | pBL1ori; Cmr; PLlacO−1:LEU4(SC)-LEU1(SC) | This study |
p29-FL | pBL1ori; Cmr; PLlacO−1: LEU1(SC) | This study |