Novel bioemulsifier produced by a Paenibacillus strain isolated from crude oil

Table 1 Effect of aeration and hydrocarbons on bioemulsifier production

Medium	ST (mN/m)	E ₂₄ (%)	[Emulsifier] (g/l)	[Biomass] (g/l)
Anaerobic (120 rpm)
MSS	52.9 ± 1.5	36.4 ± 4.4	5.0 ± 0.5	0.437 ± 0.086
MSS + Paraffin	51.1 ± 1.8	43.1 ± 3.1	5.6 ± 0.2	0.523 ± 0.043
MSS + Crude Oil	50.6 ± 1.4	42.1 ± 2.1	5.4 ± 0.4	0.496 ± 0.091
Aerobic (120 rpm)
MSS	51.1 ± 0.9	49.9 ± 2.7	6.1 ± 0.2	0.846 ± 0.112
MSS + Paraffin	50.1 ± 1.6	64.4 ± 1.6	7.9 ± 0.1	0.904 ± 0.099
MSS + Crude Oil	50.0 ± 0.6	62.1 ± 2.5	7.4 ± 0.3	0.885 ± 0.121

Surface tension values (mN/m), emulsifying activity (E₂₄, %) and biomass and bioemulsifier concentrations (g/l) obtained with Paenibacillus sp. #510 grown at 40°C under aerobic (7 days) and anaerobic (10 days) conditions in MSS and MSS supplemented with paraffin (5% w/v) or crude oil (5% w/v). The emulsifying indexes were performed using n-hexadecane. The surface tension and the emulsifying activity of MSS medium were 70.3 ± 0.4 mN/m and 0.0%, respectively. Results represent the average of three independent experiments ± standard deviation.

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