Table 1 E. coli strains and plasmids used in this study
Strain | Characteristics | Reference |
|---|---|---|
W3110 | Escherichia coli F−, λ−, INV(rrnD− rrnE)1 | ATCC 27325 |
WPJ | W3110 transformed with plasmid pJLBaroG fbr tktA | This work |
WPJRg | W3110 transformed with plasmid pJLBaroG fbr tktA and pTrcPALRg | This work |
WPJAt | W3110 transformed with plasmid pJLBaroG fbr tktA and pTrcPALAt | This work |
W(pheA−)Rg | W3110 (pheA::Km) transformed with plasmid pJLBaroG fbr tktA and pTrcPALRg | This work |
W(pheA−)At | W3110 (pheA::Km) transformed with plasmid pJLBaroG fbr tktA and pTrcPALAt | This work |
VH33 | Derivative of W3110 (ΔptsH, ptsI, crr::Km, ΔlacI, lacZ::loxP, PgalP::Ptrc) | [23] |
VPJ | VH33 transformed with plasmid pJLBaroG fbr tktA | This work |
VPJRg | VH33 transformed with plasmid pJLBaroG fbr tktA and pTrcPALRg | This work |
VPJAt | VH33 transformed with plasmid pJLBaroG fbr tktA and pTrcPALAt | This work |
Plasmids | ||
pJLBaroG fbr tktA | aroG fbr under control of the lacUV5 promoter; and tktA under its native promoter, carries lacIq and tet genes | [26] |
pTrc99A | Cloning vector, carries bla and lacIq genes and trc promoter | [27] |
pTrcPALRg | PAL gene from R. glutinis gene cloned in pTrc99A | This work |
pTrcPALAt | PAL gene from A. thaliana cloned in pTrc99A | This work |