Figure 2
PCR amplification of tannase encoding genes from several S. gallolyticus strains. (A) Amplification of 1.7-kb DNA fragment of tanA Sg with oligonucleotides 803-804. (B) Amplification of 1.4-kb tanB Sg fragment with oligonucleotides 774-775. Chromosomal DNA from the following S. gallolyticus strains was used for PCR amplification: S. gallolyticus DSM 13808 (1), S. gallolyticus subsp. gallolyticus DSM 16831T (2), S. gallolyticus subsp. gallolyticus UCN34 (3), S. gallolyticus subsp. pasteurianus DSM 15351T (4), and S. gallolyticus subsp. macedonius DSM 15879T (5). PCR fragments were subject to agarose gel electrophoresis and stained with Gel Red. Left lane, λ-EcoT14I digest (Takara). Numbers indicated some of the molecular sizes (in kb).