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Figure 2 | Microbial Cell Factories

Figure 2

From: High-quality production of human α-2,6-sialyltransferase in Pichia pastoris requires control over N-terminal truncations by host-inherent protease activities

Figure 2

Expression of N-terminally truncated ST6Gal-I variants in P.pastoris KM71H in the absence (−PI) and presence (+PI) of protease inhibitors. His-tagged enzyme variants were expressed for 96 h with (+PI) and without (−PI) protease inhibitor. WB analysis of fermentation supernatants (each 600 μL, precipitated with 20% TCA). Immunodetection with HRP conjugated anti-α2,6ST6.

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Microbial Cell Factories

ISSN: 1475-2859

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