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Table 2 Production of catechol in flask cultures by E. coli 9923 derivatives

From: Catechol biosynthesis from glucose in Escherichia coli anthranilate-overproducer strains by heterologous expression of anthranilate 1,2-dioxygenase from Pseudomonas aeruginosa PAO1

Strain

Biomass (g DCW /L)

μ (h −1 )

q Glc (g Glc /g DCW h )

q Ant (g Ant /g DCW h)

Anthranilate (g/L)

Catechol (g/L)

q Cat (g Cat /g DCW h)

Y Cat/Glc (g Cat /g Glc )

W3110 9923/ pTrc-ant3

1.69 ± 0.06

0.21 ± 0.01

0.83 ± 0.02

0.05 ± 0.01

0.00 ± 0.00

0.15 ± 0.00

0.05 ± 0.01

0.05 ± 0.00

W3110 9923 PTS/pTrc-ant3

1.58 ± 0.03

0.08 ± 0.00

0.21 ± 0.05

0.03 ± 0.01

0.22 ± 0.01

0.37 ± 0.05

0.03 ± 0.01

0.09 ± 0.01

W3110 9923/ pJLBaroG fbr tktA/pTrc-ant3

1.83 ± 0.01

0.18 ± 0.02

0.39 ± 0.07

0.07 ± 0.02

0.15 ± 0.03

0.41 ± 0.03

0.07 ± 0.02

0.10 ± 0.01

  1. Cultures were carried out in M9 mineral medium at 37°C.