Metabolic engineering and classical selection of the methylotrophic thermotolerant yeast Hansenula polymorpha for improvement of high-temperature xylose alcoholic fermentation

Table 2 Ethanol productivity, ethanol and xylitol yield of H. polymorpha transformants and control strain

Strain	Ethanol (g/l)	Ethanol yield (g/g consumed xylose)	Ethanol specific production rate (g/g biomass/h)	Ethanol productivity (g/l/h)	Xylitol (g/l)	Xylitol yield (g/g consumed xylose)
2EthOH⁻	2.054 ± 0.103	0.080 ± 0.004	0.017 ± 0.001	0.043 ± 0.002	15.657 ± 0.078	0.6074 ± 0.035
2EthOH⁻/XYL1m/XYL2	3.285 ± 0.164	0.113 ± 0.007	0.034 ± 0.002	0.083 ± 0.004	8.458 ± 0.042	0.2912 ± 0.019
2EthOH⁻/XYL1m/XYL2/XYL3	7.441 ± 0.371	0.253 ± 0.031	0.059 ± 0.003	0.142 ± 0.007	0	0
2EthOH⁻/XYL1m/XYL2/PDC1	4.732 ± 0.235	0.121 ± 0.005	0.040 ± 0.002	0.094 ± 0.005	6.751 ± 0.031	0.241 ± 0.015
2EthOH⁻/XYL1m/XYL2/XYL3/PDC1	5.043 ± 0.249	0.163 ± 0.009	0.042 ± 0.002	0.105 ± 0.005	1.104 ± 0.012	0.036 ± 0.005
2EthOH⁻/XYL1m/XYL2/XYL3/BrPA	9.817 ± 0.411	0.300 ± 0.011	0.077 ± 0.004	0.180 ± 0.009	0	0

The data are based on 7 independent replicate cultivations and ± values represent standard deviations.

ISSN: 1475-2859