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Figure 5 | Microbial Cell Factories

Figure 5

From: Physiological relation between respiration activity and heterologous expression of selected benzoylformate decarboxylase variants in Escherichia coli

Figure 5

Oxygen transfer rates of clones of E. coli SG13009 pKK233-2 expressing different BFD-variants in modified PanG mineral medium with 0.05 mg/L thiamine. (A) wild type and Leu476Pro-Ser181Thr. (B) Leu476His, Leu476Gln and Ser181Thr. Modified 250 mL shake flasks; filling volume: 10 mL; shaking frequency: 320 rpm; shaking diameter: 50 mm; temperature: 37°C, induced cultures were induced after 16 h; all OTR are shown from 6 h to enlarge the relevant part of the graphs.

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