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Figure 2 | Microbial Cell Factories

Figure 2

From: Physiological relation between respiration activity and heterologous expression of selected benzoylformate decarboxylase variants in Escherichia coli

Figure 2

Oxygen transfer rates of E. coli SG13009 pKK233-2 clones producing different BFD-variants. Cultivation at 37°C, a shaking diameter of 50 mm and different cultivation conditions in modified 250 mL shake flasks. (A) Buffered LB medium (89 mM phosphate buffer) with 10 g/L glycerol; IPTG induction at 3 h; filling volume: 25 mL; shaking frequency: 150 rpm. (B) TB medium; IPTG induction at 3.5 h; filling volume: 25 mL; shaking frequency: 150 rpm. (C) TB medium; IPTG induction at 3.5 h; filling volume: 10 mL; shaking frequency: 400 rpm.

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Microbial Cell Factories

ISSN: 1475-2859

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