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Figure 1 | Microbial Cell Factories

Figure 1

From: Physiological relation between respiration activity and heterologous expression of selected benzoylformate decarboxylase variants in Escherichia coli

Figure 1

(A) Benzoylformate decarboxylase (BFD) portion of total protein, (B) volumetric carboligation activity in crude extract and (C) optical density at 600 nm (OD 600 ) of different BFD-variants. Buffered LB medium (89 mM phosphate buffer) with 10 g/L glycerol; IPTG induction at 3 h; 250 mL shake flasks; filling volume: 25 mL; shaking frequency: 150 rpm; shaking diameter: 50 mm; temperature: 37°C.

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Microbial Cell Factories

ISSN: 1475-2859

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