Strain | Genotype/Decription | Source |
---|---|---|
L. lactis HtrA NZ9000 | MG1363 (nisRK genes on the chromosome) | [37] |
E. coli TG1 | supE thi-1 Δ(lac-proAB) Δ(mcrB-hsdSM)5 (rK- mK-) [F' traD36 proAB lacI qZ ΔM15] | ATCC |
E. coli DH5α | fhuA2 Δ(argF-lacZ)U169 phoA glnV44 Φ80 Δ(lacZ)M15 gyrA96 recA1 relA1 endA1 thi-1 hsdR17 | Invitrogen |
E. coli BL21 (DE3) | F - ompT gal dcm lon hsdS B (r B - m B - ) λ(DE3 [lacI lacUV5-T7 gene 1 ind1 sam7 nin5]) | Novagen |
Plasmid | Â | Â |
pVE5524 | Eryr, Ampr; pBS::pIL252::t trpA ::P59::rbs usp45 ::sp Usp45 -nucA-cwaM6-t1t2 | [36] |
pVE5523 | Eryr, Ampr; pBS::pIL252::t trpA ::P59::rbs usp45 ::sp Usp45 -nucA-t1t2 | [36] |
pSIP502 | Eryr; P nisA ::rbs nisA ::uidA | [66] |
pSCNIII | Cmr | J. Seegersa |
pUC19 | Ampr | [69] |
pET28(b) | Kmr | Novagen |
pSIPsp-nuc | Eryr; P nisA ::rbsnisA::spUsp45-nucA | This Work |
pUC104 | Ampr; t trpA ::P nisA ::rbs usp45 ::spUsp45-nucA | This Work |
pUC104mod | Ampr; t trpA ::P59::rbs usp45 ::spUsp45-nucA | This Work |
pUC304 | Ampr; t trpA ::P nisA ::rbs nisA ::spUsp45-nucA | This Work |
pUC504 | Ampr; t trpA ::P nisA ::rbs nisA ::spUsp45 | This Work |
pAW004 | Eryr, Ampr; pBS::pIL252::t trpA ::P59::rbs usp45 ::spUsp45-nucA-MCS-cwaM6-t1t2 | This Work |
pAW005 | Eryr, Ampr; pBS::pIL252::t trpA ::P59::rbs usp45 ::spUsp45-nucA-MCS-t1t2 | This Work |
pAW004Z | Eryr, Ampr; pBS::pIL252::t trpA ::P59::rbs usp45 ::spUsp45-nucA-lacZα-cwaM6-tlt2 | This Work |
pAW005Z | Eryr, Ampr; pBS::pIL252::t trpA ::P59::rbs usp45 ::spUsp45-nucA- lacZα-tlt2 | This Work |
pAW004ZC | Cmr, Ampr; pBS::pIL252::t trpA ::P59::rbs usp45 ::spUsp45-nucA-lacZα-cwaM6-tlt2 | This Work |
pAW005ZC | Cmr, Ampr; pBS::pIL252::t trpA ::P59::rbs usp45 ::spUsp45-nucA- lacZα-tlt2 | This Work |
pGEMc9 | Ampr; pGEMT::with cloned coh9 from cipA | This Work |
pGEMc1 | Ampr; pGEMT::with cloned coh1 from cipA | This Work |
pGEMc1-c2 | Ampr; pGEMT::with cloned coh1-coh2 from cipA | This Work |
pGEMcbm-c3 | Ampr; pGEMT::with cloned cbm3a-coh3 from cipA | This Work |
pGEMcbm | Ampr; pGEMT::with cloned cbm3a from cipA | This Work |
pAW104 | Cmr, Ampr; pBS::pIL252::t trpA ::P nisA ::rbs usp45 ::spUsp45-nucA-LacZα-cwaM6-tlt2 | This Work |
pAW105 | Cmr, Ampr; pBS::pIL252::t trpA ::P nisA ::rbs usp45 ::spUsp45-nucA-LacZα-tlt2 | This Work |
pAW301 | Cmr, Ampr; pBS::pIL252::t trpA ::P nisA ::rbs nisA ::spUsp45-nucA-cwaM6-tlt2 | This Work |
pAW302 | Cmr, Ampr; pBS::pIL252::t trpA ::P nisA ::rbs nisA ::spUsp45-nucA-tlt2 | This Work |
pAW304 | Cmr, Ampr; pBS::pIL252::t trpA ::P nisA ::rbs nisA ::spUsp45-nucA-lacZα-cwaM6-tlt2 | This Work |
pAW305 | Cmr, Ampr; pBS::pIL252::t trpA ::P nisA ::rbs nisA ::spUsp45-nucA-lacZα-tlt2 | This Work |
pAW307 | Cmr, Ampr; pBS::pIL252::t trpA ::P nisA ::rbs nisA ::spUsp45-nucA-coh9-cwaM6-tlt2 | This Work |
pAW308 | Cmr, Ampr; pBS::pIL252::t trpA ::P nisA ::rbs nisA ::spUsp45-nucA-coh9-tlt2 | This Work |
pAW310 | Cmr, Ampr; pBS::pIL252::t trpA ::P nisA ::rbs nisA ::spUsp45-nucA-coh1-cwaM6-tlt2 | This Work |
pAW311 | Cmr, Ampr; pBS::pIL252::t trpA ::P nisA ::rbs nisA ::spUsp45-nucA-coh1-tlt2 | This Work |
pAW334 | Cmr, Ampr; pBS::pIL252::t trpA ::P nisA ::rbs nisA ::spUsp45-nucA-coh1-coh2-cwaM6-tlt2 | This Work |
pAW335 | Cmr, Ampr; pBS::pIL252::t trpA ::P nisA ::rbs nisA ::spUsp45-nucA-coh1-coh2-tlt2 | This Work |
pAW328 | Cmr, Ampr; pBS::pIL252::t trpA ::P nisA ::rbs nisA ::spUsp45-nucA-cbm3a-coh3-cwaM6-tlt2 | This Work |
pAW329 | Cmr, Ampr; pBS::pIL252::t trpA ::P nisA ::rbs nisA ::spUsp45-nucA-cbm3a-coh3-tlt2 | This Work |
pAW331 | Cmr, Ampr; pBS::pIL252::t trpA ::P nisA ::rbs nisA ::spUsp45-nucA-cbm3a-cwaM6-tlt2 | This Work |
pAW332 | Cmr, Ampr; pBS::pIL252::t trpA ::P nisA ::rbs nisA ::spUsp45-nucA-cbm3a-tlt2 | This Work |
pAW504 | Cmr, Ampr; pBS::pIL252::t trpA ::P nisA ::rbs nisA ::spUsp45-lacZα-cwa M6 -tlt2 | This Work |
pAW505 | Cmr, Ampr; pBS::pIL252::t trpA ::P nisA ::rbs nisA ::spUsp45-lacZα-tlt2 | This Work |
pAW507 | Cmr, Ampr; pBS::pIL252::t trpA ::P nisA ::rbs nisA ::spUsp45-coh9-cwaM6-tlt2 | This Work |
pAW508 | Cmr, Ampr; pBS::pIL252::t trpA ::P nisA ::rbs nisA ::spUsp45-coh9-tlt2 | This Work |
pAW510 | Cmr, Ampr; pBS::pIL252::t trpA ::P nisA ::rbs nisA ::spUsp45-coh1-cwa M6 -tlt2 | This Work |
pAW511 | Cmr, Ampr; pBS::pIL252::t trpA ::P nisA ::rbs nisA ::spUsp45-coh1-tlt2 | This Work |
pAW534 | Cmr, Ampr; pBS::pIL252::t trpA ::P nisA ::rbs nisA ::spUsp45-coh1-coh2-cwaM6-tlt2 | This Work |
pAW535 | Cmr, Ampr; pBS::pIL252::t trpA ::P nisA ::rbs nisA ::spUsp45-coh1-coh2-tlt2 | This Work |
pAW528 | Cmr, Ampr; pBS::pIL252::t trpA ::P nisA ::rbs nisA ::spUsp45-cbm3a-coh3-cwaM6-tlt2 | This Work |
pAW529 | Cmr, Ampr; pBS::pIL252::t trpA ::P nisA ::rbs nisA ::spUsp45-cbm3a-coh3-tlt2 | This Work |
pAW531 | Cmr, Ampr; pBS::pIL252::t trpA ::P nisA ::rbs nisA ::spUsp45-cbm3a-cwaM6-tlt2 | This Work |
pAW532 | Cmr, Ampr; pBS::pIL252::t trpA ::P nisA ::rbs nisA ::spUsp45-cbm3a-tlt2 | This Work |
pETdock1 | Knr; pET28(b)::with cloned dock1 from celS | This Work |
pETUdock1 | Knr; pET28(b)::PT7::6xHis-uidA-dock1 | This Work |
pETU | Knr; pET28(b)::PT7::6xHis-uidA | This Work |