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Figure 9 | Microbial Cell Factories

Figure 9

From: Enhancement of solubility in Escherichia coli and purification of an aminotransferase from Sphingopyxis sp. MTA144 for deamination of hydrolyzed fumonisin B1

Figure 9

SDS-PAGE analysis of purified aminotransferase FumI. The three lanes show three neighboring fractions of C-terminally 6xHis-tagged aminotransferase FumI (FumI-HIS, 48.3 kDa) after two consecutive, preparative scale immobilized nickel affinity chromatography steps using 60-mM imidazole binding buffer. SDS-PAGE gel (12% polyacrylamide, Coomassie Brilliant Blue stained); M, molecular mass marker.

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Microbial Cell Factories

ISSN: 1475-2859

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