Production of glycoprotein vaccines in Escherichia coli

Table 1 Effect of induction on specific growth rate (μ) of glycoconjugate producing E. coli and final optical density in batch and fed-batch culture (ara = L-arabinose)

Product	Type of cultivation	Induction	OD₆₀₀at induction	μ before induction (h^-1)	μ after induction (h^-1)	final OD₆₀₀
AcrA-O1	LB batch bioreactor	ara + IPTG	0.5	1.15	0.12	0.9
AcrA-O1	LB batch bioreactor	IPTG	0.5	1.12	0.23	1.3
AcrA-O1	LB batch shake flask	ara + IPTG	0.5	1.08	0.15	1.5-2.0
AcrA-O1	LB batch shake flask	ara	0.5	0.86	0.56	2.2
AcrA-O1	Fed-batch strategy A	ara + IPTG	47	0.24	0.08	58
AcrA-O1	Fed-batch strategy B	ara + IPTG	14	0.55	0.16	59
AcrA-O1	Fed-batch strategy C	ara + IPTG	31	0.30	0.13	42
EPA-O1	LB batch shake flask	ara + IPTG	0.5	0.92	0.18	1.9
EPA-O1	Fed-batch strategy C	ara + IPTG	35	0.60	0.12	78

ISSN: 1475-2859