Steps | Insulin species (molecular mass) | Amount (mg)a | Amount (mmol)a | Recovery (%)a | Purity (%)a |
---|---|---|---|---|---|
Culture broth b | Insulin precursor 7041 Da | 3075 | 0.437 | 100 | 20 |
Chelating sepharose FF c | Insulin precursor 7041 Da | 2921 | 0.415 | 95 | 96 |
Desalting & Lyophilization | Insulin precursor 7041 Da | 2859 | 0.406 | 93 | 98 |
Transpeptidation | Pre-insulin 5920 Da | n.d. d | n.d. d | n.d. d | n.d. d |
Resource RPC Desalting & Lyophilization | Pre-insulin 5920 Da | 1915 | 0.323 | 74 | 90 |
Deprotection | Human insulin 5808 Da | n.d. d | n.d. d | n.d. d | n.d. d |
Resource RPC (two passages), Desalting & Lyophilization | Human insulin 5808 Da | 1537 | 0.264 | 60 | 99 |