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Figure 3 | Microbial Cell Factories

Figure 3

From: Application of simple fed-batch technique to high-level secretory production of insulin precursor using Pichia pastoris with subsequent purification and conversion to human insulin

Figure 3

Time course analysis of secretory IP production. Cell-free culture supernatants were analyzed by 10% Tricine SDS-PAGE from samples taken directly before the addition of methanol (time 0) and 24, 48, 72, 96, 120 and 144 hours after the onset of methanol feeding. The arrow denotes the position of IP and M the lane of the molecular weight marker.

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Microbial Cell Factories

ISSN: 1475-2859

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