Figure 4From: A highly efficient pipeline for protein expression in Leishmania tarentolae using infrared fluorescence protein as markerOnline detection of IFP in L. tarentolae. IFP-His expressing Leishmania cells were grown in 25 cm2-tissue culture flasks in 10 mL of BHI medium in static upright (SU), static flat (SF) and dynamic (60 rpm) flat (A) position. ANAC42-His expressing cells were used as negative control. After 0, 6, 24, 48, 72 and 96 h of incubation 100 μL-samples were IR-scanned in the wells of a 96-well microtiter plate.Back to article page