Strain/derivative | Relevant characteristics | Reference |
---|---|---|
E. coli JM101 | supE, thi, Δ(lac-proAB), F' | [45] |
E. coli JM101 aroK- | E. coli JM101 aroK Δ::cm | This work |
E. coli aroB - | E. coli K12 strain BW25113 ΔaroB::kan (JW3352) | [48] |
E. coli aroE - | E. coli K12 strain BW25113 ΔaroE::kan (JW3242) | [48] |
E. coli PB28 | PB12 ΔpykA::cat ΔpykF::gen | [31] |
E. coli PB12 | JM101 Δ(ptsH-I-crr)::kan glc+ | [18] |
PB12.SA1 | PB12 ΔaroL | This work |
PB12.SA11 | PB12.SA1 pJLBaroGfbr tktA pTOPOaroB | This work |
PB12.SA2 | PB12 ΔaroL ΔaroK::cm | This work |
PB12.SA21 | PB12.SA2 pJLBaroGfbr tktA pTOPOaroB | This work |
PB12.SA22 | PB12.SA2 JLBaroGfbr tktA pTOPOaroB aroE | This work |
PB12.SA3 | PB12.SA2 ΔpykF::gen | This work |
PB12.SA31 | PB12.SA3 pJLBaroGfbr tktA pTOPOaroB aroE | This work |
PB12.SA4 | PB12.SA2 ΔpykA::gen | This work |
PB12.SA41 | PB12.SA4 pJLBaroGfbr tktA pTOPOaroB aroE | This work |
E. coli TOP10 | F- mcrA Δ(mrr-hsd RMS-mcrBC) φ 80lacZ ΔM15 ΔlacX 74 recA 1 ara D139 Δ(ara-leu)7697 galU galK rpsL end A1nupG | Invitrogen |
Plasmids | ||
pJLBaroGfbr tktA | pJLBaroGfbr (aroGfbr expressed from the lacUV5 promoter, lacI q and tet genes (Tcr), pACYC184 replication origin) derivative, containing the tktA gene with its native promoter. | |
pCR®-Blunt II-TOPO® | Plac lacZ- α ORF T7 promoter ccdB kan (Kmr) Zeocin pUC origin. | Invitrogen |
pTOPO aroB | pCR®-Blunt II-TOPO® containing the aroB gene | This work |
pTOPO aroB aroE | pTOPOaroB derivative containing the aroB and aroE genes | This work |