Induction | Culture supernatant | Periplasmic Space | Cytoplasm | Total | ||||
---|---|---|---|---|---|---|---|---|
4 hr | Ovn | 4 hr | Ovn | 4 hr | Ovn | 4 hr | ovn | |
0.1 mM IPTG | ||||||||
Total Activity (U/L) | 8,659 | 15,076 | 13,509 | 9,983 | 15,189 | 24,502 | 37,357 | 49,561 |
Total Protein (mg/L) | 147 | 170 | 44.5 | 17.8 | 186 | 163 | 378 | 351 |
Sp. Activity (U/L) | 58.8 | 89.0 | 304 | 560 | 81.7 | 150 | 99.0 | 141 |
0.5 mM IPTG | ||||||||
Total Activity (U/L) | 8,437 | 14,171 | 19,649 | 13,488 | 10,589 | 24,480 | 38,675 | 52,139 |
Total Protein (mg/L) | 137 | 156 | 45.0 | 20.5 | 137 | 199 | 319 | 376 |
Sp. Activity (U/L) | 61.7 | 91.1 | 437 | 660 | 77.3 | 123 | 121 | 139 |
1.0 mM IPTG | ||||||||
Total Activity (U/L) | 13,561 | 14,402 | 17,647 | 13,232 | 24,502 | 26,632 | 55,710 | 54,266 |
Total Protein (mg/L) | 153 | 175 | 39.5 | 20.2 | 163 | 168 | 356 | 363 |
Sp. Activity (U/L) | 88.9 | 82.4 | 447 | 656 | 150 | 159 | 157 | 149 |