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Table 3 Important parameters for the fed-batch fermentation of pCOLD III/POx at 25°C.

From: Evaluation of different expression systems for the heterologous expression of pyranose 2-oxidase from Trametes multicolor in E. coli

 

Batch phase

fed-batch phase

DCW

5.7

28.9

Yx/s

0.55

0.47

μmax [h-1]

0.24

0.15

vol. activity [U/L]

440

3300

process time [h]

20

16

productivity [U/L·h]

22

206

space time yield [mg/L·h]

2.8

25.8

  1. Protein expression was induced by addition of lactose (0.5% w/v) from the start of the cultivation.
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Microbial Cell Factories

ISSN: 1475-2859

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