Evaluation of different expression systems for the heterologous expression of pyranose 2-oxidase from Trametes multicolor in E. coli

Table 2 Growth parameters and results of batch fermentations at different temperatures using the pCOLD III/POx expression system.

fermentation	1	2	3	4	5	6
OD₆₀₀ at induction	0.5	2.5	0.5	2.5	0.5	2.5
temp. [°C]	25	25	20	20	15	15
DCW [g/L]	4.9	5.0	4.8	4.6	4.4	4.6
Yx/s [g/g]	0.47	0.49	0.46	0.47	0.43	0.45
μ_max [h^-1]	0.23	0.22	0.11	0.11	0.06	0.05
vol. activity [U/L]	330	190	360	110	580	220
process time [h]	17	13	31	15	49	28
productivity [U/L·h]	19	15	12	7	12	8
space time yield [mg/L·h]	2.4	1.9	1.5	0.9	1.5	1

Protein expression was induced by providing 5 g/L (0.5% w/v) lactose at different times.

ISSN: 1475-2859