Figure 3

SDS-PAGE analysis of IB formation during fermentations at different temperatures with the pET21d⁺/POx expression system. After lysis, cell debris containing IB was collected by centrifugation; 100 mg cell debris were washed, resuspended in Laemmli Buffer, heated at 95°C for 5 min and 10 μl aliquots were loaded per lane. Lane 1, 37°C; lane 2, 30°C; lane 3, 25°C; lane 4, molecular mass marker proteins; lane 5, 20°C.